Advantages of CelCoatTM

Broader Solvency Range

Target to solvent/stationary phase interaction is a driving force in analytical or prep chiral work. But, it is also the major driving force for good solubility, vital for any preparative/process chromatography. An expanded dynamic resolution range of HPLC eluent solvency and improved relationship between target solvency and eluent, are but a couple of the major advantages of CelCoat™ for chiral method development. For the first time in chiral HPLC there is now a degree of predictability between target parameters and elution solvent choice. As a result, our product finally allows the use of generic gradients in chiral separations. Although already common in non-chiral method development for analytical, prep and process chromatography for normal and reverse phase, generic gradients have yet to become common in chiral HPLC, but with CelCoat™ they will become ever more used for chiral separations as well.

CelCoat™ has also been designed to work exceptionally well when used with mixed polar eluents in normal phase and polar organic mode. Examples of such polar modifier mixes are 50/50 IPA/EtOH, IPA/MeOH, EtOH/MeOH etc. These mixes allow chromatographers to maximize eluent choice and enable multiple, high solubility options for chiral prep. Due to CelCoat™’s greater resolving power over that of historic phases or clones, as is common with reverse phase, chromatographers can now reduce column costs, amount of solvent used and time taken to achieve chiral separations through the use of short, 75 mm length columns for analytical CelCoat™ chiral HPLC.



Therefore, A Better Correlation to Polarity

CelCoat™ was designed by us to allow similar polarities of target compounds, to be eluted over a much wider range of polarities than the historic coated cellulose chiral phases, and thereby greatly improve the selectivity and resolution capabilities.

Less Dependency on Buffers = Your Choice

CelCoat™ has also been designed to minimize the dependency on buffers. Historic or clone cellulose coated chiral phases require the use of buffers for the separation of ionic compounds such as propanolol and pindolol. With CelCoat™, gradients with 100% IPA or 100% EtOH as the “B” solvent, or, better still using for the “B” solvent; 50% IPA/50% EtOH or 50% IPA/50% MeOH (both from 0.3% to 100% polar organic) or 50% EtOH/50% MeOH (limited to 0.3% to 50% polar organic owing to the limited solubility of EtOH and MeOH in Hexane) can give excellent resolution and peak shapes with NO USE OF BUFFER even for very polar targets as indicated in Chomatograms A & B above.
As shown in Chromatogram C below, however, adding buffer to 100% IPA can massively improve both resolution and peak shape, as it will for other organic polar modifier mixes. But with CelCoat™, it is often a CHOICE not AN ESSENTIAL


Higher Efficiencies (P/M)

CelCoat™ was also Enhanced™ by us to give higher efficiencies (40,000 – 60,000 plates/m) for certain targets as indicated in Chromatogram D below. Such higher efficiencies further allows the shorter 75 mm length columns to be used to reduce chiral column costs, amount of solvent used and time taken to achieve rapid chiral separations.


Higher Backpressure Capabilities

CelCoat™ also offers greater pressure stability (>2000 psi) than historic coated cellulose phases, which can allow extended generic gradients at 1 ml/min for even 5 micron, 4.6 x 250 HPLC columns, from 0.3 to 100% polar modifier, when using the preferred mixed IPA/EtOH or IPA/MeOH or 0.3 to 50% with EtOH/MeOH as the polar modifier.

Normal, Polar Organic or Reverse Phase – Only One Column to Buy for Universal Application

While CelCoat™ is shipped ready for use with normal phase solvents; our product can be easily converted for use in reverse phase solvents. There is now no need to buy two columns, one for normal phase and another for reverse phase. You chose when to convert from normal to reverse phase. Conversion to aqueous reverse phase is simply achieved by washing with either 100% IPA or 50% IPA/50% EtOH for approximately 30 minutes at 1 ml/min. We do, however, suggest that once converted for use as a reverse phase column, you do not switching back to normal phase.


Available in a Format to Suit Your Needs

CelCoat™ is available from stock packed in 4.6x250mm HPLC columns in wide pore, spherical 5 micron phase. Or, you may special order other dimensions (50 to 250mm lengths and 2.1 to 21mm id’s) or the same wide pore material in larger particle sizes (10, 20 and 20-45 micron).

Bulk phase is available to custom order in wide pore, spherical 10, 20 and 20-45 micron. We can also provide, to custom order 20 and 20-45 micron sizes in a patented, axial plus radial compression, pre-packed cartridge format in 125, 250, 400, 800, 1400 & 2800 gram cartridges for process HPLC chiral preparations. So the same wide pore, spherical silica based CelCoat™, is available from 2.1 to 21 mm id standard stainless steel column HPLC range up to multi-kilo, patented, radial and axial compression, process HPLC columns. There is now no need for you to be exposed to the hazards of small particle silica based products when doing large-scale laboratory or process preparations.

AECS-QuikPrep™ is your analytical, preparative and process chromatography specialist. Unlike the historic refusal of certain brands to sell their cellulose-coated phases as 10 micron bulk phase, we will sell CelCoat™ 10 micron at prices competitive with historic bulk phase price for 20 micron. If you still require 20 micron, even when 10 micron is available, we will sell you that also at highly competitive prices.



You may now be able to consider using CelCoat™’s unique selectivity for non-chiral separations and also in chiral Flash Chromatography; two more interesting possibility from AECS QuikPrep™?

If you are interested in trying CelCoat™, please email or phone us to discuss your specific needs. If CelCoat™ RBC-1 is not suitable maybe one of the several other chiral phases we have in development might be better. On signing mutually agreeable Confidentiality Agreements, we will be delighted to collaborate with you to resolve your research analytical, laboratory or process scale chiral preparation.

We can also synthesize target compounds, purify natural and synthetic complex mixtures or design and produce for you a custom HPLC or Flash phase. Why not describe your requirements and request our collaboration?

Conclusion

The QuikPrep CelCoat™ is an evolution of historic cellulose coated silica based, chiral HPLC phases. The selectivity of CelCoat™ would be somewhat similar to historic ranges of cellulose coated silica columns, but CelCoat™ has it’s own unique selectivity designed to decrease the dependence on use of buffer and increase the dynamic solvent strength range for the same target solvency range. Methods developed on earlier, similar generic classification of chiral columns, would need their solvent ratio compositions re-worked, but our research suggests superior resolution is often achieved, after a very rapid, generic, re-optimisation procedure.